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Image Search Results
Journal: MethodsX
Article Title: A knowledge-driven protocol for prediction of proteins of interest with an emphasis on biosynthetic pathways
doi: 10.1016/j.mex.2020.101053
Figure Lengend Snippet: A case study to demonstrate the enzyme mining protocol. A) Quercetin biosynthesis pathway (chalconeflavanone isomerase (CHI)). B) Homologue search using EC number of enzyme in UniProt database. C) Functional ImportantResidue mapping (residue block) to identify true hits (grey colour) from the alignment of homologues and the alignment usedfor Jumpstart PSI-BLAST search. D) FIR mapping (residue block) and phylogenetic analysis performed to identify true enzymehits (grey colour) from our proteome. E) The identified hit were further validated using tissue-based quantification by qRT-PCR(bars) and transcriptome analysis (line).
Article Snippet: C) Functional ImportantResidue mapping (residue block) to identify true hits (grey colour) from the alignment of homologues and the
Techniques: Functional Assay, Residue, Blocking Assay, Quantitative RT-PCR